Smad4 sc-7966

WebbSmad4 (N-16): sc-1908. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization. Try Smad4 (B-8): sc-7966 or Smad4 (DCS-46): sc- … WebbSanta Cruz Biotechnology anti smad4 Anti Smad4, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. …

(PDF) Smad4 and p53 synergize in suppressing ... - ResearchGate

Webb24 aug. 2013 · All incubations with anti-SMAD4 antibody (B-8, sc-7966, Santa Cruz, USA) at 1:200 dilutions were carried out overnight at 4 °C. Then, the specimens were briefly washed in PBS (3×5 min) and incubated at room temperature with anti-rabbit antibody and then streptavidin–biotin protein peroxidase (Maixin Fuzhou, China). WebbTo establish the Smad4-expressing BxPC3 cells, we transfected 2μg pcDNA3 Flag-Smad4 vector to BxPC3 cells and selected as described previously. (2)The control cell line B3EV was established as described previously. (8) RNA interference. RNA interference against Smad4 has been described previously. shania rebecca https://myagentandrea.com

Supplementary tables and figures Table S1. Patient and tumor ...

Webb23 jan. 2004 · Smad proteins play key roles in intracellular signaling of the transforming growth factor-β (TGF-β) superfamily. E1A, an adenoviral oncoprotein, is known to inhibit TGF-β-induced transactivation through binding to Smad proteins. Webb2 juni 2011 · Mouse anti-Smad4 (1:300, clone Sc-7966; Santa Cruz Biotechnology) and rabbit anti-Hoxa9 (1:300, clone 07-178; Upstate Biotechnology) antibodies were applied … WebbSuitable control antibody: Smad4 Antibody (B-8): sc-7966 is recommended as control antibody for monitoring of Smad4 expression knockdown by Western blotting or immunofluorescence Get details about si/shRNA Gene Silencers Gene silencers are intended for noncommercial and research use only. Click here for details. See product … polygon 4.3 torrent

Smad4 (B-8) Antibody sc-7966 from Santa Cruz Biotechnology, Inc …

Category:Cancer Management and Research Volume 13 - Dove Press Open …

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Smad4 sc-7966

SMAD4 Loss triggers the phenotypic changes of pancreatic ductal ...

Webb25 mars 2024 · Smad4 Antibody (B-8) is a mouse monoclonal IgG 1 κ Smad4 antibody, cited in 799 publications, provided at 200 µg/ml. raised against amino acids 1-552 …

Smad4 sc-7966

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Webb25 maj 2024 · SMAD4, a key regulator of transforming growth factor-β (TGF-β) signaling, plays a major role in cell growth, migration, and apoptosis. In particular, TGF-β/SMAD … Webb8 mars 2024 · Mouse anti-Smad4 (sc-7966) and rabbit anti-Smad1/5/8 (sc-6031) were purchased from Santa Cruz Biotechnology (Dallas, TX). 2.3.4 Alkaline phosphatase assay Alkaline phosphatase (ALP) activity was measured via ALP staining in C2C12 cells transfected with SMAD6 variants.

WebbSmad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to TGF-beta signaling. ... (GTX112980) diluted at 1:1000 … WebbSAN TA C RUZ BI OTEC HNOL OG Y, INC . Smad4 (N-16): sc-1908 SantaCruzBiotechnology,Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com BACKGROUND Smad proteins, the mammalian homologs of the Drosophila mothers against dpp (Mad) have been …

WebbSmad4 (B-8) is available conjugated to agarose (sc-7966 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; to HRP (sc-7966 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; to either … WebbWestern blot was performed to detect the knockout effect using SMAD4 antibody (Santa Cruz Biotechnology, Inc. sc-7966). The PaCa DD183 cell line was derived from a …

Webbquantification of Smad1, Smad2, and Smad4 levels in 6 month old prostates from indicated genotypes. (E) Colony outgrowth in arrested . Phlpp1. null MEFs after infection with …

Webb11 mars 2024 · Background: Smad4 and p53 mutations are the most common mutations in human colorectal cancers (CRCs). We evaluated whether and how they are synergistic in intestinal carcinogenesis using novel... shania peoples choice awardsWebbSmad4 (sc-7966), Santa Cruz Biotechnology (Santa Cruz, CA). Protein lysates of the OSCC cell lines were prepared in EBC buffer (50 mM Tris-HCl, 120 mM sodium chloride (NaCl), … shania photo shootWebb14 maj 2010 · The chromatin solution was subjected to immunoprecipitation using a OneDay ChIP Kit (Diagenode, Sparta, NJ) according to the manufacturer's instructions. … shania real nameWebb24 aug. 2013 · All incubations with anti-SMAD4 antibody (B-8, sc-7966, Santa Cruz, USA) at 1:200 dilutions were carried out overnight at 4 °C. Then, the specimens were briefly … shania poneyWebb12 juli 2012 · AGS cells with and without SMAD4 knock-down were co-cultured with H. pylori or BMP2 to assess the expression of BMP ... mouse monoclonal anti-SOX2 (1:4500, Sigma-Aldrich), mouse monoclonal anti-SMAD4 (sc-7966, 1:1000, Santa Cruz Biotechnology), rabbit polyclonal anti-pSMAD1/5/8 (1:1000, Cell Signalling), rabbit … shania photosWebb6 maj 2024 · Here we show that, upon activation, AR forms a transcriptional complex with SMAD4 to orchestrate a muscle hypertrophy programme by modulating SMAD4 … shania redditWebb1 juli 2013 · Because Smad4 is a central signaling molecule of TGF-β–induced signal transduction, we hypothesized that nestin expression status might depend on TGF-β1/Smad4 signaling. Nestin promoted tumor cell migration and induced EMT in PDAC We investigated whether nestin played a direct functional role in facilitating PDAC cell … shania plastic surgery