Eea1 molecular weight
Web1/2000 - 1/10000. Predicted molecular weight: 162 kDa. Notes; IP Use at 2-5 µg/mg of lysate. WB 1/2000 - 1/10000. Predicted molecular weight: 162 kDa. Target. Function. ... EEA1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using … WebSeveral polypeptides have been shown to complex with E1A proteins, directly or indirectly (Fig. 1, Tables 2 and 3).Those most consistently and readily found include proteins with …
Eea1 molecular weight
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WebMar 1, 1999 · Abstract EEA1, an early-endosomal protein originally identified as an autoantigen, is essential for endocytic membrane fusion. It interacts with early endosomes via binding to the membrane lipid phosphatidylinositol 3-phosphate (PtdIns3P) and the active form of the small GTPase Rab5. WebEEA1 (E-8) is available conjugated to agarose (sc-365652 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; to HRP (sc-365652 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; to either …
WebFeb 4, 2000 · Early endosome antigen 1 (EEA1) is 170-kDa polypeptide required for endosome fusion. EEA1 binds to both phosphtidylinositol 3-phosphate (PtdIns3P) and to Rab5-GTP in vitro, but the functional role of this dual interaction at the endosomal membrane is unclear. WebMay 10, 2011 · The EEA1-containing high-molecular-weight complexes might constitute both EEA1 homodimer as well as EEA1 heterooligomers that contained other EEA1-interacting proteins.
WebBackbone manufacturer Clontech Backbone size w/o insert (bp) 4700 Vector type Mammalian Expression Selectable markers Neomycin (select with G418) Growth in Bacteria Bacterial Resistance (s) Kanamycin, 50 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number High Copy Gene/Insert Gene/Insert name RAB7 (Homo … WebJun 2, 1995 · We have cloned a human cDNA encoding an evolutionarily conserved 180-kDa protein on early endosomes named EEA1 (Early Endosome Antigen1). EEA1 is associated with early endosomes since it co-localizes by immunofluorescence with the transferrin receptor and with Rab5 but not with Rab7. Immunoelectron microscopy…
WebOct 13, 2024 · EEA1 is an early endosomal Rab5 effector protein that has been implicated in the docking of incoming endocytic vesicles before fusion with early endosomes. Because of the presence of complex endosomal …
WebMar 30, 2024 · Even though the depletion of VPS35 increased the size of the EEA1-labeled early endosomes, in line with SNX1 pattern, the endosomal actin patches were intact in the VPS35 KO cell line. ... (Molecular Probes, 1 μL per 22 × 22 mm coverslip) was added to the secondary antibody step. All coverslips were then mounted onto Mowiol supplemented … crush instant drink mixWebApr 10, 2024 · The mode of transport depends on the physical and chemical properties of the substances to be transported, including molecular weight, lipid solubility, plasma protein binding, and cerebral blood flow. Passive transport is limited to small-sized molecules that have sufficient lipid solubility such as gaseous oxygen and carbon dioxide … bulacan to sm auraWebMay 1, 2024 · Early endosomes, regarded as the main sorting station on endocytic pathway, are characterized by high frequency of homotypic fusions mediated by tethering protein EEA1. Despite intensive investigations, biogenesis of endosomes, boundaries between early and late endosomes, and process of cargo transition though them remain obscure. … crush instrumentalWebThis Anti-EEA1 Antibody is validated for use in WB, IC for the detection of EEA1. Western Blot (SNAP ID) Analysis: 1:250 dilution from a previous lot detected EEA1 on 10 µg of C2C12 cell lysate. Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected EEA1 in HeLa, A431, and C2C12 cells. crushin songWebEarly endosomal antigen 1 (EEA1) is a 162 kDa membrane bound protein component specific to the early endosome and is essential for fusion between early endocytic vesicles. Early endosomes are cytoplasmic compartments which fuse with endocytic vesicles for redistribution of extracellular compounds to alternate destinations. crush instagramWebView Rabbit Polyclonal anti-EEA1 Antibody (NBP1-30914). Validated Applications: WB, ICC/IF, IHC, IHC-P, IM. Validated Species: Human, Mouse, Rat + more. ... The observed … crush insomniac ticketsWebEEA1 is a hydrophilic peripheral membrane protein present in cytosol and membrane fractions. It partitions in the aqueous phase after Triton X-114 solubilization and is … bulacan travel restrictions